E-MTAB-1608 - Transcription profiling by array of Drosophila melanogaster male flies to identify transcriptome changes during the heat hardening process
Released on 1 May 2014, last updated on 3 June 2014
A common measure of heat resistance in Drosophila is the time it takes a fly to be knocked down by higher temperatures measured in a long knockdown tube or in small glass vials. This heat knockdown process involves gradual paralysis and non-responsiveness to stimuli, usually within half hour duration of constant heat exposure, and is ostensibly reversible upon return to lower temperatures, although there may be long-term fitness effects. Hardening for increased heat resistance requires exposure periods of up to 3 hours at temperatures ranging from 34C to 38C. The experiment was aimed at understanding the heat hardening process and samples were taken during the hardening period (15 minutes, 45 minutes, 1½ hours, 2¼ hours and 3 hours).
transcription profiling by array, co-expression, growth condition design, in vivo, replicate design, stimulus or stress design, time series design