E-MTAB-1598 - Transcription profiling by array of of Drosophila melanogaster halo[AJ] snail[Df(2L)TE116GW11] and halo[AJ] sna[V2] homozygous mutant embryos at two timepoints of embryogenesis

Status
Released on 10 January 2014, last updated on 3 June 2014
Organism
Drosophila melanogaster
Samples (1)
Array (1)
Protocols (6)
Description
Expression profiling of Drosophila melanogaster halo[AJ] snail[Df(2L)TE116GW11] and halo[AJ] sna[V2] homozygous mutant embryos at two timepoints of embryogenesis. Embryos were manually sorted and assayed at cellularisation stage (stage 5 to early stage 6) and at gastrulation stage (late stage 6 to early stage 8). Homozygous mutant embryos were sorted based on their reduced transparency caused by the recessive mutation of halo[AJ]. Stage-matched halo[AJ] embryos served as wildtype control. Total RNA was extracted, amplified and hybridized to Affymetrix GeneChip Drosophila Genome array version 2. Three biological replicates at each condition were generated.
Experiment types
transcription profiling by array, development or differentiation design, genetic modification design, individual genetic characteristics, reference design, replicate design, time series design
Contact
Citation
A conserved role for Snail as a potentiator of active transcription. Rembold M, Ciglar L, Yáñez-Cuna JO, Zinzen RP, Girardot C, Jain A, Welte MA, Stark A, Leptin M, Furlong EE. Genes & Development  (2014)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-1598.idf.txt
Sample and data relationshipE-MTAB-1598.sdrf.txt
Raw data (1)E-MTAB-1598.raw.1.zip
Array designA-AFFY-35.adf.txt
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