E-MTAB-1587 - RNA-seq of coding RNA from sheep oocytes and granulosa cells from each follicle stage to investigate global gene expression during early ovarian folliculogenesis

Status
Released on 29 January 2014, last updated on 3 May 2014
Organism
Ovis aries
Samples (35)
Protocols (7)
Description
Separate transcription profiling of oocytes and granulosa cells for each follicle stage: primordial (PD), primary (PM), secondary (SC) follicles and the small antral stage (SA) obtained by Laser Capture Microdissection (LCM) and RNAseq. The purpose of this study was to describe global gene expression during early ovarian folliculogenesis for each follicular compartment, to identify differential and specific gene expression between the 2 follicular compartments and during follicular development, to investigate specific function and pathways and to explore bi-directional communication between oocytes and GC.
Experiment types
RNA-seq of coding RNA, cell type comparison design, co-expression, development or differentiation design
Contact
Citations
An overview of gene expression dynamics during sheep early ovarian folliculogenesis, Part II: Ovarian follicular development. Agnes Bonnet , Cedric Cabau , Olivier Bouchez , Julien sarry , Nathalie Marsaud, Florent Woloszyn , Philippe Mulsant , Beatrice Mandon-Pepin.
An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog. Bonnet A, Cabau C, Bouchez O, Sarry J, Marsaud N, Foissac S, Woloszyn F, Mulsant P, Mandon-Pepin B. :904 (2013), Europe PMC 24350644
An overview of gene expression dynamics during sheep early ovarian folliculogenesis, Part I: Specificity of follicular compartments and bi-directional dialog. Agnes Bonnet , Cedric Cabau , Olivier Bouchez , Julien sarry , Nathalie Marsaud, Florent Woloszyn , Philippe Mulsant , Beatrice Mandon-Pepin.
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-1587.idf.txt
Sample and data relationshipE-MTAB-1587.sdrf.txt
Links