5 protocols
Title: Affymetrix CEL analysis. Description:
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
nucleic acid hybridization to array protocol
P-AFFY-3 GeneChip hybridization
nucleic acid extraction protocol
Standard Affymetrix protocol for tota RNA isolation
growth protocol
Cells from spleen were prepared for flow cytometry as previously described (Chatzidakis, I et al., 2007. An essential role for tnf in modulating thresholds for survival, activation, and tolerance of cd8+ t cells. J Immunol 178:6735-45)