6 protocols
normalization data transformation protocol
Quantile normalized using R
nucleic acid labeling protocol
Illumina TotalPrep RNA Amplifikation Kit
culture protocol
Standard adherent growth in Hams F12 (Life Technologies), 5 % FCS (Life Technologies), 5 ug / ml bovine Insulin (Sigma), 1 ug / ml hydrocortisone (Sigma)
nucleic acid extraction protocol
Using Rneasy Mini Kit (qiagen) according to manufacturers instructions
culture protocol
Cultering under suspension conditions in low-attachment flasks (Corning) in MammoCult (stemcell technologies) following the MammoCultss instructions