high throughput sequence alignment protocol
Trinity was used to perform the de novo assembly of high-quality clean reads.
nucleic acid extraction protocol
Total RNA was extracted from matural plant and seedling of Reaumuria soongorica with E.Z.N.A. Plant RNA Kit (Omega Bio-tek, Doraville, GA, USA)
Materials were sampled from wild field. Seedling was harvested two weeks after germination.
nucleic acid library construction protocol
The two RNA samples from different developmental stages were pooled equally to construct the cDNA library.
nucleic acid sequencing protocol
The paired-end library with insert sizes of 200 C700 bp was sequenced.