E-MTAB-1542 - Transcription profiling by array of D. melanogaster 3rd instar larva normal and EPN infected to investigate immune response
Released on 7 March 2014, last updated on 3 June 2014
Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves as well for expanding nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system we used before to show the importance of haemolymph clotting and eicosanoids during the infection. We used the well-established tripartite model (Drosophila, nematodes, bacteria), DNA chips and bioinformatic tools to compare gene expression in non-infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria. Infected (GFP positive) larvae were collected 6 hours after infection.
transcription profiling by array, co-expression, disease state, in vivo, replicate
The Drosophila immune response towards entomopathogenic nematodes and their associated bacteria. Badrul Arefin, Lucie Kucerova, Pavel Dobes, Robert Markus, Zhi Wang, Hynek Strnad, Pavel Hyrsl, Michal Zurovec, Ulrich Theopold.