Title: Affymetrix CEL analysis. Description:
nucleic acid hybridization to array protocol
P-AFFY-3 GeneChip hybridization
WT-Ovation RNA Amplification System (NuGEN Technologies Inc., San Carlos, CA, USA). The reaction products were purified with a QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany).
(Parameters: Amplification = PCR, Mass unit = Micro gram)
Frozen PAXgeneTM Blood RNA tubes were thawed at room temperature for 3 hours, followed by total RNA extraction with the PAXgeneTM Blood RNA kit according to the manufacturerﾒs instruction.
(Parameters: Extracted product = total_RNA, Amplification = none)
For each collection, 2.5 ml of peripheral blood was drawn into a PAXgene Blood RNA tube (PreAnalytiX GmbH, Hombrechtikon, CH) and stored at -80 degrees C.