6 protocols
AccessionType
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
nucleic acid hybridization to array protocol
P-AFFY-3 GeneChip hybridization
bioassay_data_transformation
RMA
grow
Arabidopsis thaliana seedlings were grown for 7 days in vitro on 1/2 MS media; seedlings were placed on nylon mesh to facilitate transfer without touching the plants, long-day conditions 16/8 with 22 degrees C/20 degrees C, transfer at day7 either to control media or 0.2mM 3MB containg media, plants were grown on this new media until harvest either after 24h, 48h, 96h or 168h
nucleic_acid_extraction
RNA extraction with RNAeasy of samples stored in RNAlater
specified_biomaterial_action
0.2mM 3MB added to the 1/2MS media