E-MTAB-1464 - Transcription profiling by array of mouse ATDC5 cells with induced differentiation in the absence or presence of EGR1 over a 72-hour time-course
Released on 7 May 2013, last updated on 3 May 2014
Progression through chondrogenesis is driven by interaction with the microenvironment. Cells respond to changes in the microenvironment by altering their transcriptome. The mechanisms connecting environmental cues to chromatin and signaling factors involved in early epigenomic remodeling are poorly understood. We here study the role of the early growth factor response gene EGR1 and its interaction with Polycomb Group epigenetic regulators in chondrogenesis. Chondrogenic differentiation was induced in ATDC5 cells in the absence (RNAi) or presence of EGR1; RNA samples for microarray gene expression profiling were collected at t=0, 2, 4.5, 8,16, 24, 72 hrs post-induction of differentiation.
transcription profiling by array, cellular modification, co-expression, development or differentiation, genetic modification, in vitro, replicate, time series
The immediate early gene product EGR1 and polycomb group proteins interact in epigenetic programming during chondrogenesis. Spaapen F, van den Akker GG, Caron MM, Prickaerts P, Rofel C, Dahlmans VE, Surtel DA, Paulis Y, Schweizer F, Welting TJ, Eijssen LM, Voncken JW. PLoS One 8(3):e58083 (2013), PMID:23483971