6 protocols
AccessionType
image_acquisition
Scanning was carried out according to the manufacturer's instructions.
labeling
Labelling and hybridisation to Agilent G4413A arrays were carried out according to the manufacturer's instructions.
hybridization
Labelling and hybridisation to Agilent G4813A arrays were carried out according to the manufacturer's instructions.
specified_biomaterial_action
Cell pellets were briefly resuspended in synthetic complete media without glucose and then dispensed into 96 well plates preloaded with fresh media containing glucose and a perturbing agent to yield final concentrations of 2% glucose (SC only control), 0.
grow
Overnight cultures were diluted to OD600 0.1 and allowed to undergo at least two doublings in synthetic complete media before harvesting by centrifugation.
nucleic_acid_extraction
RNA was isolated using the MasterPure Yeast RNA kit MPY03100.