E-MTAB-1451 - ChIP-chip by array of Streptomyces coelicolor A3(2) to determine AbrC3 binding sites
Released on 4 January 2014, last updated on 3 May 2014
Streptomyces coelicolor A3(2)
ChIP on chip assay of AbrC3 to determine its binding sites in S. coelicolor M145 genome using purified anti-AbrC3 polyclonal antibodies. Samples from nutrient broth (NB) cultures were collected at 36, 48 and 60 hours, cross-linked, and finally hybridized to DNA 104K microarrays designed by University of Surrey.
ChIP-chip by array, binding site identification, dye swap, time series
Deciphering the regulon of Streptomyces coelicolor AbrC3, a positive response regulator of antibiotic production. Rico S, Santamaría RI, Yepes A, Rodríguez H, Laing E, Bucca G, Smith CP, Díaz M. Appl Environ Microbiol 80(8):2417-2428 (2014), Europe PMC 24509929