5 protocols
AccessionType
hybridization
As per manufacturers protocol on the Illumina Omni 2.5M BeadChip
labelling
As per manufacturers protocol on the Illumina Omni 2.5M BeadChip
bioassay_data_transformation
Genotype calling was performed using GenomeStudio 2010.3 from Illumina Inc. using standard settings. Due to the restricted number of samples pre-calculated gentoype clusters from a larger cohort were used, as provided by Illumina (HumanOmni2.5-4v1_D.egt). FinalReports including genotypes and underlying signals were exported from GenomeStudio into reference genome FORWARD orientation (build hg19) based on HumanOmni2.5-4v1_D.bpm.
nucleic_acid_extraction
DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen).
sample treatment protocol
Peripheral blood was extracted and white blood cell fractions separated.