Affymetrix Generic Hybridization
Gene level normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
Log2 normalized expression signal
Equal amounts of RNA extracted from 3 mice from the same group were combined as a pool. For all samples, 300 ng of total RNA was used to perform target preparation using the Whole Transcript Sense Target Labeling Protocol procedure (Affymetrix, High Wycombe, UK).
Housing 2 weeks 12h Light:12h Dark. Dissection at Zeitgeber Time 12
Trizol-Chloroform. RNAqueous kit