6 protocols
AccessionType
hybridization protocol
Affymetrix Generic Hybridization
bioassay_data_transformation
Gene level normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
bioassay_data_transformation
Log2 normalized expression signal
labeling
Equal amounts of RNA extracted from 3 mice from the same group were combined as a pool. For all samples, 300 ng of total RNA was used to perform target preparation using the Whole Transcript Sense Target Labeling Protocol procedure (Affymetrix, High Wycombe, UK).
grow
Housing 2 weeks 12h Light:12h Dark. Dissection at Zeitgeber Time 12
nucleic_acid_extraction
Trizol-Chloroform. RNAqueous kit