6 protocols
AccessionNameType
P-MTAB-30391
hybridization
Hybridization was performed using mouse genome CGH microarray244K from Agilent Technologies (Santa Clara, CA) according to the manufacturer's protocol. Slides were hybridized for 48 hours.
P-MTAB-30392
image_acquisition
Arrays were scanned with an Agilent microarray scanner. Raw data was then extracted using Agilent Feature Extraction. All following data analysis was performed in R using Bioconductor free packages (http://www.bioconductor.org).
P-MTAB-30387
grow
Mice were housed in standard clean conditions and were aged until moribund
P-MTAB-30388
specified_biomaterial_action
Thymocytes and tails were isolated from moribund mutant animals, red blood cells lysed (thymocytes), pelleted and stored at -80 degrees prior to gDNA extraction.
P-MTAB-30390
labeling
DNAs were labeled with Cy3 orCy5 fluorescent accordingto BioPrime array CGH genomic labeling protocol (Invitrogen, Carlsbad, CA) and cleaned using purelink pcr purification kit (invitrogen).
P-MTAB-30389
nucleic_acid_extraction
Genomic DNA from diseased thymocytes and tail matched samples was extracted using standard methods.