8 protocols
AccessionNameType
P-MTAB-30291
bioassay_data_transformation
Detection P values <0.01 were used to filter all data. Significant ?2 differential expression called on Log2 transformed data with an adjusted P value if <0.005
P-MTAB-30290
bioassay_data_transformation
Array probe summaries were calculated in Beadstudio and quantile normalised
P-MTAB-30287
labeling
Labelled with Ambion Total Prep RNA amplification Kit.
P-MTAB-30285
specified_biomaterial_action
Thymocytes were isolated from wt and mutant animals, red blood cells lysed and stained for TCRb and CD5 cell surface antigens and the TCRb/CD5low populations flow sorted, pelleted and stored at -80 degrees prior to total RNA extraction.
P-MTAB-30284
grow
Mice were housed in standard clean conditions and were used at 2-3 weeks of age
P-MTAB-30286
nucleic_acid_extraction
Standard Trizol reagent extraction
(Parameters: Extracted product = total_RNA, Amplification = none)
P-MTAB-30289
image_acquisition
Scanned in Illumina BeadArray Reader using BeadStudio
P-MTAB-30288
hybridization
Hybridised at 58 C for 16 hours in oven with rocking platform