Outgrowth endothelial cells were isolated from the mononuclear fraction of umbilical cord blood and cultured on collagen coated flasks. Once OEC colonies emerged they were expanded in culture to study cell growth kinetics. OECs were sampled at an early and late passage.
Standard Illumina scanning protocol
Standard Illumina hybridization protocol
Total RNA was extracted using RNAqueous kit (Ambion)