6 protocols
AccessionType
bioassay_data_transformation
Quantile normalisation
grow
Outgrowth endothelial cells were isolated from the mononuclear fraction of umbilical cord blood and cultured on collagen coated flasks. Once OEC colonies emerged they were expanded in culture to study cell growth kinetics. OECs were sampled at an early and late passage.
labeling
Biotin
image_acquisition
Standard Illumina scanning protocol
hybridization
Standard Illumina hybridization protocol
nucleic_acid_extraction
Total RNA was extracted using RNAqueous kit (Ambion)