8 protocols
AccessionType
labeling
cDNA Synthesis: AFFYmetrix kit 900812 ; cDNA Cleanup: AFFYmetrix kit 900812 ; cRNA Synthesis: AFFYmetrix kit 900812 ; cRNA Cleanup: AFFYmetrix kit 900812 ; Spike Target Element: mRNA+Target ; Spiking Control: Affymetrix standard ; Dye: biotin
grow
LB (Luria-Bertani) exponential growth phase
specified_biomaterial_action
Drug (siderophore sulfactam BAL30072) added at 4 mg/l
nucleic_acid_extraction
Nucleic Acid Extraction Method: unknown ; Nucleic Acid Type: total_RNA ; Amplification Method: None
bioassay_data_transformation
Data Processing/Normalization:MAS5 normalization
feature_extraction
Image Analysis Software: GCOS ; Image Analysis Algorithm: GCOS
hybridization
Washing/Staining Procedure: FS450_0007 ; Washing/Staining Instrumentation: Fluidics Station 450
image_acquisition
Scanner Hardware: Affymetrix GeneChip Scanner 3000 ; Scanning Software: GCOS