E-MTAB-1368 - ChIP-seq of human MCF-7 cells with siRNA-knocked down Transducin-like enhancer protein 1 (TLE1) and treated with estrogen to study TLE1-mediated estrogen receptor-chromatin interactions
Released on 14 November 2012, last updated on 20 November 2012
Estrogen receptor (ER) binds to distal enhancers within the genome and requires additional factors, such as the Forkhead protein FoxA1, for mediating chromatin interactions. We now show that the human Groucho protein, Transducin-like enhancer protein 1 (TLE1), positively assists some ER-chromatin interactions, a role that is distinct from its general role as a transcriptional repressor. We show that specific silencing of TLE1 inhibits the ability of ER to bind to a subset of ER binding sites within the genome, a phenomenon that results in perturbations in phospho-RNA Pol II recruitment. Furthermore, TLE1 is essential for effective ER-mediated cell division. We have discovered a distinct role for TLE1, as a necessary transcriptional component of the ER complex, where it facilitates ER-chromatin interactions.
ChIP-seq, ChiP-seq, compound treatment, replicate
Transducin-like enhancer protein 1 mediates estrogen receptor binding and transcriptional activity in breast cancer cells. Holmes KA; Hurtado A; Brown GD; Launchbury R; Ross-Innes CS; Hadfield J; Odom DT; Carroll JS.