Hybridization with Affymetrix protocol EukGe-WS2v4 by using the GeneChip Fluidics Station 450
GC-RMA algorithm and a Cyber-T statistical test for pair comparisons
cDNA using the MessageAmp II-Biotin Enhanced kit from Ambion
Samples were grown on MS3 media 3% sucrose for 7 days, prior to the dawn of the 8th day, they were collected. Col-0 wild type and tic-2 mutant, a T-DNA insertion, were used. 12:12 photoperiod, samplig took place 10 minutes before dawn of the 8th day. 2 biological replicates per genotype were performed.
Total RNA extraction
Sample collection 10 minutes before dawn on the 8th day of growth