7 protocols
AccessionType
data acquisition
The intensity files were loaded into GenomeStudio v.2010.3 software for quality control and gene expression analysis
scanning
BeadChips were dried and subsequently scanned with an Illumina BeadArray Reader
hybridization
Hybridization reactions were performed for 18 hours on Illumina HumanHT12 v.4.0 Expression BeadChips
labeling
Total RNA were fluorescently labelled, amplified and hybridized in triplicate
treatment
K-562 cells were used to produce five distinct cell cultures: wild type, FHC-silenced, FHC-reconstituted,wild type hemin-treated, FHC-silenced hemin-treated
extraction
Cells were lysed and RNA was extracted using TRIzol method, according to manufacturer's instruction
grow
K-562 cells were cultured in standard growth condition