E-MTAB-1336 - Transcription profiling by array of erythromyeloid K-562 cells silenced or not silenced for FHC gene upon hemin treatment
Released on 16 October 2013, last updated on 3 May 2014
K-562 cells were cultured in standard growth conditions and used to produce five distinct cell cultures: wild type, FHC-silenced,wild type hemin-treated, FHC-silenced hemin-treated. Then the cells were lysed and RNA was extracted using TRIzol method.Total RNA were fluorescently labelled, amplified and hybridized in triplicate for 18 hours on Illumina HumanHT12 v.4.0 Expression BeadChips and after scanning, analysis was performed with GenomeStudio v.2010.3 software, for quality control and mRNA expression analysis.
transcription profiling by array, cellular process
Identification of H ferritin-dependent and independent genes in K562 differentiating cells by targeted gene silencing and expression profiling. Misaggi R, Di Sanzo M, Cosentino C, Bond HM, Scumaci D, Romeo F, Stellato C, Giurato G, Weisz A, Quaresima B, Barni T, Amato F, Viglietto G, Morrone G, Cuda G, Faniello MC, Costanzo F. Gene 13 (2013), Europe PMC 24239552