E-MTAB-1331 - Transcription profiling by array of human W12 cell lines transfected with HPV16 siRNA as a model to study cervical neoplastic progression

Status
Released on 12 August 2013, last updated on 3 May 2014
Organism
Homo sapiens
Samples (36)
Array (1)
Protocols (8)
Description
We utilised our in vitro model of cervical neoplastic progression, W12, to investigate the effect of HPV16 viral oncogene depletion on well-defined integrant- and episome- associated series. To target HPV16 viral oncogenes we used our previously published E7-targeting siRNA sequence that caused substantial depletion of both E7 and E6 in CaSki cells. We found all E7-siRNA treated W12 series underwent widespread autophagy and senescence, with up-regulation of an innate immune response.
Experiment types
transcription profiling by array, in vitro, is expressed
Contact
Citation
Depletion of HPV16 early genes induces autophagy and senescence in a cervical carcinogenesis model, regardless of viral physical state. JE Hanning, HS Saini, MJ Murray, MM Caffarel, S van Dongen, DM Ward, EM Barker, CG Scarpini, IJ Groves, MA Stanley, AJ Enright, MR Pett, N Coleman. J Pathol  (2013)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-1331.idf.txt
Sample and data relationshipE-MTAB-1331.sdrf.txt
Raw data (1)E-MTAB-1331.raw.1.zip
Processed data (1)E-MTAB-1331.processed.1.zip
Array designA-MEXP-2072.adf.txt
Links