E-MTAB-1331 - Transcription profiling by array of human W12 cell lines transfected with HPV16 siRNA as a model to study cervical neoplastic progression
Released on 12 August 2013, last updated on 3 May 2014
We utilised our in vitro model of cervical neoplastic progression, W12, to investigate the effect of HPV16 viral oncogene depletion on well-defined integrant- and episome- associated series. To target HPV16 viral oncogenes we used our previously published E7-targeting siRNA sequence that caused substantial depletion of both E7 and E6 in CaSki cells. We found all E7-siRNA treated W12 series underwent widespread autophagy and senescence, with up-regulation of an innate immune response.
transcription profiling by array, in vitro, is expressed
Depletion of HPV16 early genes induces autophagy and senescence in a cervical carcinogenesis model, regardless of viral physical state. JE Hanning, HS Saini, MJ Murray, MM Caffarel, S van Dongen, DM Ward, EM Barker, CG Scarpini, IJ Groves, MA Stanley, AJ Enright, MR Pett, N Coleman. J Pathol (2013)