E-MTAB-132 - Transcription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1

Status
Submitted on 7 June 2007, released on 1 January 2010, last updated on 3 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expression of Pdx1 and established Pdx1 targets was confirmed. The RNA was then submitted for labeling and hybridization to the PancChip cDNA microarray.
Experiment types
transcription profiling by array, dye swap, genetic modification
Contacts
Citation
Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress. Sachdeva MM, Claiborn KC, Khoo C, Yang J, Groff DN, Mirmira RG, Stoffers DA.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-132.idf.txt
Sample and data relationshipE-MTAB-132.sdrf.txt
Raw data (1)E-MTAB-132.raw.1.zip
Processed data (2)E-MTAB-132.processed.1.zip, E-MTAB-132.processed.2.zip
Array designA-CBIL-10.adf.txt
R ExpressionSetE-MTAB-132.eSet.r
Links