E-MTAB-132 - Transcription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1
Submitted on 7 June 2007, released on 1 January 2010, last updated on 3 May 2014
In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expression of Pdx1 and established Pdx1 targets was confirmed. The RNA was then submitted for labeling and hybridization to the PancChip cDNA microarray.
transcription profiling by array, dye swap, genetic modification
David Groff <firstname.lastname@example.org>, Cynthia Khoo <email@example.com>, Doris Stoffers <firstname.lastname@example.org>, Jonathan Schug <email@example.com>, Kathryn Claiborn <firstname.lastname@example.org>, Mira Sachdeva <email@example.com>, Peter White <firstname.lastname@example.org>, Raghavendra Mirmira <email@example.com>
Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress. Sachdeva MM, Claiborn KC, Khoo C, Yang J, Groff DN, Mirmira RG, Stoffers DA.