E-MTAB-1274 - High-resolution methylation profiling of glioblastoma neural stem cells and reprogrammed derivatives
Released on 15 March 2013, last updated on 23 September 2017
Glioblastoma-derived neural stem (GNS) cells were reprogrammed to induced pluripotent stem (iPS) cells by transgenic expression of OCT4 and KLF4. Genome-wide DNA methylation status was profiled at 485,000 loci to assess epigenetic erasure and restoration due to reprogramming and redifferentiation to the neural stem (NS) cell state.
methylation profiling by array, disease state, replicate
Widespread resetting of DNA methylation in glioblastoma-initiating cells suppresses malignant cellular behavior in a lineage-dependent manner. Stefan H. Stricker, Andrew Feber, Pär G. Engström, Helena Carén, Kathreena M. Kurian, Yasuhiro Takashima, Colin Watts, Michael Way, Peter Dirks, Paul Bertone, Austin Smith, Stephan Beck, Steven M. Pollard. Genes and Development 27(6):654-69 (2013), Europe PMC 23512659