E-MTAB-1101 - Transcription profiling by array of human macrophages infected with clinical isolates of Mycobacterium avium

Status
Released on 10 February 2012, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (6)
Description
Mycobacterium avium is a significant opportunistic intracellular pathogen in humans, pigs and birds. Human and porcine infection with M. avium is mainly due to M. avium subsp. hominissuis, whereas birds are typically infected with M. avium subsp. avium. Reasons for this distribution of subspecies between the hosts are uncertain, but they might be due to differences in exposure or to different abilities to infect the respective hosts. We wanted to assay how isolates of the two subspecies interact with a human host in a macrophage model to gain more knowledge on possible differences in virulence. This study compares clinical isolates of M. avium with regards to their ability to invade and replicate intracellularly in human monocytes/macrophages and to the gene expression triggered in these cells upon infection. Further focus in the description of protocols is limited to the parts of the study that regards the expression analysis.
Experiment types
transcription profiling by array, co-expression, in vitro, pathogenicity, replicate
Contact
Citations
Intracellular growth of Mycobacterium avium subspecies and global transcriptional responses in human macrophages after infection. Agdestein A, Jones A, Flatberg A, Johansen TB, Heffernan IA, Djonne B, Bosco A, Olsen I. BMC Genomics 15(1):58 (2014), PMID:24450835
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-1101.idf.txt
Sample and data relationshipE-MTAB-1101.sdrf.txt
Raw data (1)E-MTAB-1101.raw.1.zip
Array designA-MEXP-1171.adf.txt
Links