DNaseI fragmentation and biotinylation with terminal transferase.
Scanner type: GeneChip 3000 7G. Scanning software: default software
Modified tiling array Affy target Hybridization with GeneChip control oligoB2, Affymetrix.
To extract total RNA yeast cells were lysed by vortexing with glass beads in the presence of hot acid phenol. RNA was then cleaned by phenol:chlorophorm extraction followed by ethanol precipitation.