4 protocols
AccessionType
labeling
DNaseI fragmentation and biotinylation with terminal transferase.
scanning
Scanner type: GeneChip 3000 7G. Scanning software: default software
hybridization
Modified tiling array Affy target Hybridization with GeneChip control oligoB2, Affymetrix.
nucleic_acid_extraction
To extract total RNA yeast cells were lysed by vortexing with glass beads in the presence of hot acid phenol. RNA was then cleaned by phenol:chlorophorm extraction followed by ethanol precipitation.