E-MIMR-341 - Transcription profiling of human CD4CD45RO T-cells stimulated with interleukin-2, interleukin-6 or TNF-alpha and harvested at 24, 72 or 144 hours or stimulated with anti-CD3 antibodies and harvested at 24 hours

Status
Released on 1 June 2008, last updated on 12 October 2011
Organism
Homo sapiens
Samples (1)
Array (1)
Protocols (21)
Description
Blood taken from a healthy human donor was used to isolate CD4CD45RO T-cells. Purified cells were stimulated with either interleukin-2 (IL-2), Interleukin-6 (IL-6) and TNF-alpha and harvested at 24 hours, 72 hours (3 days) and 144 hours (6 days) or anti-CD3 antibodies and harvested at 24 hours. These were compared to unstimulated purified CD4CD45RO T-cells harvested at 0 hours.
Experiment types
transcription profiling by array, stimulated type, time series
Contacts
Citation
Cytokine stimulation of T lymphocytes regulates their capacity to induce monocyte of tumour necrosis factor-alpha but not in interleukin-10: possible relevance to pathophysiology of rheumatoid arthritis. Parry, S.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MIMR-341.idf.txt
Sample and data relationshipE-MIMR-341.sdrf.txt
Raw data (1)E-MIMR-341.raw.1.zip
Processed data (1)E-MIMR-341.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-MIMR-341.eSet.r
Links