E-MEXP-98 - Transcription profiling by array of Arabidopsis mutant for hsf1 and hsf3 after heat shock treatment
Released on 15 December 2004, last updated on 3 May 2014
The aim of the expression profiling was to define and to apply criteria that allow discrimination between the effects of heat stress (HS) and HSF-dependent gene expression in Arabidopsis. Therefore we determined and analyzed the expression profiles of wild type (WT) and homozygous hsf1--/hsf3-- double knock out mutant plants (abbreviated hsf1/3). Hsf1/3 plants are deficient in HSF1 and HSF3 activity and unable to induce the fast transient expression of mRNAs of a number of HS genes tested (Lohmann et al., 2004).
The effects of HS on gene expression were analyzed after one hour heat treatment (37C) of leaf tissue.
transcription profiling by array, stimulus or stress
Identification of novel Heat Shock Factor dependent genes and biochemical pathways in Arabidopsis thaliana. Busch W, Wunderlich M, Schöffl F. , Europe PMC 15610345