E-MEXP-91 - Transcription profiling of human tamoxifen treated myeloid leukemia K562-MERT cells
Released on 30 June 2004, last updated on 3 June 2014
K562 cells, a myeloid leukemia cells line, were engineered to express a tamoxifen inducible dominant negative Myb (MERT). K562-MERT cells were cultured for 3 days in the absence and presence of 1 uM tamoxifen. The RNA was then extracted from the untreated and tamoxifen treated K562-MERT cells and submitted to Incyte Genomics for poly(A) RNA selection, probe preparation, hybridization of the labeled cDNA to the micorarray chip (Incyte Genomics) and data analysis.
transcription profiling by array, transcript identification