E-MEXP-903 - Transcription profiling by array of human glioma cells after treatment with TGF-beta or LY2109761
Released on 16 May 2007, last updated on 2 May 2014
In order to identify the genes that are regulated by TGF-beta in glioma, we serum starved two glioma cell lines, U373MG and U87MG, for 16h and we treated them with vehicle,100pM TGF-beta, 2uM inhibitor of the TGF-beta Receptor I(TbRI)(LY2109761), or both 100pM TGF-beta plus 2uM TbRI for 3h. Then, cell were collected and total RNA was extracted.
transcription profiling by array, compound treatment
Joan Seoane <email@example.com>, Alberto A Ocaña, Alejandra A Bruna, Alexandra A Arias, Avelina A Tortosa, Federico F Rojo, Jaume J Mora, Jose J Baselga, Rachel R Darken, Raquel R Paris, Silvia S Peñuelas
High TGFbeta-Smad activity confers poor prognosis in glioma patients and promotes cell proliferation depending on the methylation of the PDGF-B gene. Bruna A, Darken RS, Rojo F, Ocaña A, Peñuelas S, Arias A, Paris R, Tortosa A, Mora J, Baselga J, Seoane J. , Europe PMC 17292826
Expression analysis of all protease genes reveals cathepsin K to be overexpressed in glioblastoma. Verbovšek U, Motaln H, Rotter A, Atai NA, Gruden K, Van Noorden CJ, Lah TT. :e111819 (2014), Europe PMC 25356585