E-MEXP-79 - Transcription profiling of QMP pheromone-mediated gene expression in the honey bee brain

Status
Released on 1 May 2004, last updated on 2 May 2014
Organism
Apis mellifera
Samples (8)
Array (1)
Protocols (9)
Description
This experiment was designed to monitor the gene expression changes of young bees raised with and without queen mandibular pheromone (QMP). This experiment was a timecourse, comparing QMP- to QMP+ bees over 4 days of exposure. Bees that had eclosed over 16 hours were collected from a brood frame and placed in cages, 35 bees/cage. 8 hours later, queen mandibular pheromone (QMP, 0.1 queen equivalents) treatment was initiated, and fresh QMP was placed in the cage every day. One hour after QMP treatment had been refreshed, QMP- and QMP+ cages were collected into liquid nitrogen. 8 cages of each were collected on each day of the timecourse (1 day of treatment = Day 1, as well as Day 2, Day 3, and Day4).
Experiment types
transcription profiling by array, compound treatment, dye swap, replicate, time series
Contacts
Charles W Whitfield <charlie@life.uiuc.edu>, Christina M Grozinger, Gene E Robinson, Noura M Sharabash
Citation
Pheromone-mediated gene expression in the honey bee brain. Grozinger, Christina M.; Sharabash, Noura M.; Whitfield, Charles W.; Robinson, Gene E.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-79.idf.txt
Sample and data relationshipE-MEXP-79.sdrf.txt
Array designA-MEXP-36.adf.txt
Links