E-MEXP-79 - Transcription profiling of QMP pheromone-mediated gene expression in the honey bee brain
Released on 1 May 2004, last updated on 2 May 2014
This experiment was designed to monitor the gene expression changes of young bees raised with and without queen mandibular pheromone (QMP). This experiment was a timecourse, comparing QMP- to QMP+ bees over 4 days of exposure. Bees that had eclosed over 16 hours were collected from a brood frame and placed in cages, 35 bees/cage. 8 hours later, queen mandibular pheromone (QMP, 0.1 queen equivalents) treatment was initiated, and fresh QMP was placed in the cage every day. One hour after QMP treatment had been refreshed, QMP- and QMP+ cages were collected into liquid nitrogen. 8 cages of each were collected on each day of the timecourse (1 day of treatment = Day 1, as well as Day 2, Day 3, and Day4).
transcription profiling by array, compound treatment, dye swap, replicate, time series
Charles W Whitfield <email@example.com>, Christina M Grozinger, Gene E Robinson, Noura M Sharabash
Pheromone-mediated gene expression in the honey bee brain. Grozinger, Christina M.; Sharabash, Noura M.; Whitfield, Charles W.; Robinson, Gene E.