E-MEXP-78 - Transcription profiling of human mammary epithelial cell line MDA-MB-468 acking endogenous Smad4 nfected with either an adenovirus carrying GFP cDNA (control) or Smad4 cDNA (to restore the Smad4 activity in the cells). Cells were treated with TGF-b1 (0.5 ng/ml) or BMP-7 (300 ng/ml) for 2 or 12h

Status
Submitted on 27 January 2004, released on 15 March 2004, last updated on 2 May 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (17)
Description
Identification of novel TGF-b1 and BMP-7-regulated genes in epithelial cells.

Human mammary epithelial cell line MDA-MB-468 that is lacking endogenous Smad4 was infected with either an adenovirus carrying GFP cDNA (control) or Smad4 cDNA (to restore the Smad4 activity in the cells). Cells were treated with TGF-b1 (0.5 ng/ml) or BMP-7 (300 ng/ml) for 2 or 12h. Smad4-independent vs Smad4-dependent response was measured. Also response to both growth factors was compared.
Experiment types
transcription profiling by array, cell type comparison, compound treatment, time series
Contacts
Marcin Kowanetz <Marcin.Kowanetz@licr.uu.se>, Aristidis Moustakas, Carl-Henrik Heldin, Rosita Bergström, Ulrich Valcourt
Citations
Arthritis suppression by NADPH activation operates through an interferon-beta pathway. Olofsson P, Nerstedt A, Hultqvist M, Nilsson EC, Andersson S, Bergelin A, Holmdahl R. :19 (2007), PMID:17490473
Id2 and Id3 Define the Potency of Cell Proliferation and Differentiation Responses to Transforming Growth Factor {beta} and Bone Morphogenetic Protein. Kowanetz, Marcin; Valcourt, Ulrich; Bergstrom, Rosita; Heldin, Carl-Henrik; Moustakas, Aristidis. Mol Cell Biol 24(10):4241 (2004), PMID:15121845
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-78.idf.txt
Sample and data relationshipE-MEXP-78.sdrf.txt
Raw data (1)E-MEXP-78.raw.1.zip
Array designA-SNGR-5.adf.txt
R ExpressionSetE-MEXP-78.eSet.r