E-MEXP-720 - Transcription profiling of stationary phase E.coli wild type and crl::cat mutant strains to identify Crl-regulated genes
Submitted on 22 May 2006, released on 1 January 2007, last updated on 2 May 2014
We wished to identify Crl-regulated genes in stationary phase in E.coli, and whether those overlap with the previously identified regulon of RpoS. Therefore wildtype E.coli (MC4100) and its isogenic crl::cat mutant were grown at 30oC. Total RNA was extracted at on OD (578nm) of 4 (during entry into stationary phase) and then the analysis proceeded as described in detail in the protocoles. The experiment was repeated three times and the results from those experiments are presented here.
transcription profiling by array, genetic modification
Nassos Typas <email@example.com>, Athanasios Typas, Claudia Barembruch, Regine Hengge
Stationary phase reorganisation of E.coli transcription machinery vy Crl protein, a fine tuner if sigmaS activity and levels. Athanasios Typas; Claudia Barembruch; Regine Hengge. EMBO J