E-MEXP-71 - Transcription profiling of light and carbon signaling interactions in Arabidopsis
Released on 23 January 2004, last updated on 27 March 2012
A. thaliana seeds of Columbia (Col-0) ecotype were surface-sterilized, plated on designated media and vernalized for 48 hours at 8C. Plants were grown semi-hydroponically under 16-hr-light, 8-hr-dark cycles at a constant temperature of 23C on basal Murashige and Skoog medium supplemented with 2 mM KNO3, 2 mM NH4NO3 and 30 mM sucrose. Two-week-old seedlings were transferred to fresh MS media without nitrogen and sucrose and dark-adapted for 48 hours. To perform specific metabolic treatments (-C-L, +C-L, -C+L, +C+L), two-week-old seedlings were transferred to fresh MS medium containing 0% or 1% sucrose and either placed back into the dark or illuminated with white light for an additional 8 hours. The various treatments were compared to one another using the treatment of -C-L as the background treatment to which all other treatments (-C+L, +C-L, +C+L) were compared.
transcription profiling by array, growth condition
Karen E Thum <email@example.com>, Andrei Kouranov, Gloria M Coruzzi, Michael J Shin, Peter M Palenchar
Genome-wide investigation of light and carbon signaling interactions in Arabidopsis. Karen Thum; Michael Shin; Peter Palenchar; Andrei Kouranov; Gloria Coruzzi.