E-MEXP-566 - Transcription profiling of primary human tubular epithelial cells (HUTEC) response to TGFbeta at three concentrations to study the epithelial to mesenchymal transition
Submitted on 9 February 2006, released on 31 December 2006, last updated on 2 May 2014
This experiment was aimed to study the Epithelial to mesenchimal transition (EMT) induced by TGFbeta in primary tubular epithelial cells. Primary cultures were grown for 4 days using three different TGFbeta1 concentrations: 5 ng/ml, 10 ng/ml and 50 ng/ml. Cells were grown in plastic flasks (Falcon) or in plastic flasks (Falcon) covered by type I collagen. Differentially expressed genes were identified comparing treated vs. control cells maintained for 4 days in 1% serum without TGFbeta1.
transcription profiling by array, dose response
Genes involved in TGF beta1-driven epithelial-mesenchymal transition of renal epithelial cells are topologically related in the human interactome map. Campanaro S, Picelli S, Torregrossa R, Colluto L, Ceol M, Del Prete D, DAngelo A, Valle G, Anglani F. BMC Genomics 8:383 (2007), Europe PMC 17953753