E-MEXP-489 - Transcription profiling of an orthotopic mouse model of lung cancer vs control mice and A549 cells in culture
Submitted on 9 November 2005, released on 1 March 2006, last updated on 3 May 2014
Homo sapiens, Mus musculus
An orthotopic model of lung cancer was established in Rag-2 null mice by injecting 1 x 106 GFP-A549 cells into the mouse lung via the trachea. After 6 weeks a primary tumor develops in the right lung. RNA was collected from tumors established in the orthotopic model after 6 weeks in three separate mice and from the right lung of three sham control mice that were injected with PBS by the same method. RNA was also collected from the A549 cells in culture. RNA was applied to an Affymetrix Customized Protease Microarray (HuMu ProtIn chip). This custom chip is able to distinguish human and mouse genes by using oligonucleotides specific for each species. The chip contains all known human and mouse proteases and protease inhibitors. A distribution of gene expression from a comparison of all human and mouse genes present in the tumor vs. the control lung was indentified as well as a distribution of gene expression from a comparison of all human and mouse genes present in the tumor vs. the cells in culture. Human and mouse genes differentially expressed in each group were identifed.
transcription profiling by array, species
Analysis of host- and tumor-derived proteases using a custom dual species microarray reveals a protective role for stromal MMP12 in non-small cell lung cancer. Cancer Res