E-MEXP-471 - Chromatin immunoprecipitation of human cell lines with oestrogen receptor (ER), the E2F transcription factor 4 (E2F4),
Released on 1 May 2006, last updated on 3 May 2014
The Goal of the experiment was to validate the predicted regulatory modules identified by an algorithm.For this we took advantage of the the genome-wide location analysis technique (or ?ChIP/chip?). We selected modules predicted to be bound by the estrogen receptor (ER), the E2F transcription factor 4 (E2F4), the signal transducer and activator of transcription 3 (STAT3), and the hypoxia-inducible factor 1 HIF1), to print a DNA microarray. In the current study, the microarray was then probed by ChIP/chip for ER and E2F4.
ChIP-chip by array, binding site identification, unknown experiment type
Fran�ois Robert <Francois.Robert@ircm.qc.ca>, Alain R Bataille, Benoit Coulombe, Christian Poitras, CŽline Lef�bvre, Dominique Bergeron, Genevi�ve Deblois, JosŽe Lagani�re, Mathieu Blanchette, Vincent Ferretti, Vincent Gigu�re, Xiaoyu Chen
Genome-wide computational prediction of transcriptional regulatory modules reveals new insights into human gene expression. Mathieu Blanchette; Alain Bataille; Xiaoyu Chen; Christian Poitras; JosŽe Lagani�re; CŽline Lef�bvre; Genevi�ve Deblois; Vincent Gigu�re; Vincent Ferretti; Dominique Bergeron; Benoit Coulombe; Fran�ois Robert.