E-MEXP-463 - Transcription profiling of E. coli LZ41 and LZ54 strains containing drug-resistant alleles of different topoisomerase genes treated with norfloxacin

Status
Released on 5 November 2005, last updated on 3 May 2014
Organism
Escherichia coli
Samples (6)
Array (1)
Protocols (8)
Description
Effect of supercoiling level on gene expression using LZ41 and LZ54 strains containing drug-resistant alleles of different topoisomerase genes. LZ41 strain treatment with norfloxacin strongly relaxes DNA, whereas in LZ54 strain the same treatment generates high negative supercoiling (Khodursky et al., 1995, PNAS 92:11801-5; Ziechedrich et al, 1997, Genes Dev. 11:2580-92).
Experiment types
 growth condition, strain or line, transcription profiling by array
Contacts
Georgi Muskhelishvili <g.muskhelishvili@jacobs-university.de>, Andrew Travers, Marcel Geertz, Nicolas Blot, Ramesh Mavathur
Citations
Analog regulation of metabolic demand. Sonnenschein N, Geertz M, Muskhelishvili G, Hütt MT. BMC Syst Biol 5:40 (2011), Europe PMC 21406074
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-463.idf.txt
Sample and data relationshipE-MEXP-463.sdrf.txt
Raw data (1)E-MEXP-463.raw.1.zip
Processed data (1)E-MEXP-463.processed.1.zip
Array designA-MEXP-129.adf.txt
Links