E-MEXP-462 - Transcription profiling of E. coli LZ41 and LZ54 strains containing drug-resistant alleles of different topoisomerase genes treated with norfloxacin

Status
Released on 1 November 2005, last updated on 3 May 2014
Organism
Escherichia coli
Samples (8)
Array (1)
Protocols (8)
Description
Effect of FIS and H-NS on gene expression at relexed and hypernagative supercoiling level using LZ41 and LZ54 strains. LZ41 and LZ54 strains contain drug-resistant alleles of different topoisomerase genes. LZ41 strain treatment norfloxacin strongly relaxes DNA, whereas in LZ54 strain the same treatment generates high negative supercoiling (Khodursky et al., 1995, PNAS 92:11801-5; Ziechedrich et al, 1997, Genes Dev. 11:2580-92).
Experiment types
 dye swap, strain or line, transcription profiling by array
Contacts
Georgi Muskhelishvili <g.muskhelishvili@jacobs-university.de>, Andrew Travers, Marcel Geertz, Nicolas Blot, Ramesh Mavathur
Citations
Analog regulation of metabolic demand. Sonnenschein N, Geertz M, Muskhelishvili G, Hütt MT. BMC Syst Biol 5:40 (2011)
Analog regulation of metabolic demand. Sonnenschein N, Geertz M, Muskhelishvili G, Hütt MT. BMC Syst Biol 5:40 (2011), Europe PMC 21406074
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-462.idf.txt
Sample and data relationshipE-MEXP-462.sdrf.txt
Raw data (1)E-MEXP-462.raw.1.zip
Processed data (1)E-MEXP-462.processed.1.zip
Array designA-MEXP-129.adf.txt
Links