E-MEXP-457 - Transcription profiling of individual resistant and infected epidermalsingle cells of the susceptible barley line Pallas
Released on 13 November 2005, last updated on 3 June 2014
Investigation of the interaction between barley and B. graminis. Individual resistant and infected epidermal cells of the susceptible barley line Pallas were easily distinguished by microscopy, and their contents collected in micro-capillaries. We hybridized 12 arrays with radioactively labeled material from these cell specific samples; 3 with material from resistant cells, 3 with material from infected cells, 3 with material from non-inoculated control cells, and 3 identical samples for technical replication. Pooling equal amounts of material from the 3 infected samples made these last 3 samples.
disease state, transcription profiling
Peter H Hagedorn <email@example.com>, Hans H Thordal-Christensen, Michael F M Lyngkjær, Patrick P Schweizer, Torben T Gjetting