E-MEXP-413 - Transcription profiling of E47 targets in the neuroblastoma cell line SK-N-SH at 8 hours and 20 hours

Released on 1 February 2006, last updated on 3 May 2014
Homo sapiens
Samples (5)
Arrays (2)
Protocols (9)
E47 is a basic Helix Loop Helix (bHLH) transcription factor that has important roles in cell fate determination and differentiation of many cell types. In the nervous system E47 heterodimerizes with tissue-specific, pro-neural bHLH transcription factors and activates downstream target genes. To identify the relevant target genes of bHLH transcription factors in neural cells, we performed gene expression profiling of the human neuroblastoma cell line SK-N-SH engineered to acutely express ectopic E47 by an adenoviral vector. The experiments were done at two time points following adenoviral infection, 8 hours and 20 hours. Genes induced by E47 after 8 hours are likely to be direct targets of this transcription factor.
Experiment types
transcription profiling by array, genetic modification
Degradation of Id2 by the anaphase-promoting complex couples cell cycle exit and axonal growth. Anna Lasorella, Judith Stegmuller, Daniele Guardavaccaro, Guangchao Liu, Maria S. Carro, Gerson Rothschild, Luis de la Torre-Ubieta, Michele Pagano, Azad Bonni, Antonio Iavarone.
Investigation descriptionE-MEXP-413.idf.txt
Sample and data relationshipE-MEXP-413.sdrf.txt
Raw data (1)E-MEXP-413.raw.1.zip
Processed data (1)E-MEXP-413.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-34.adf.txt
R ExpressionSetE-MEXP-413.eSet.r