8 protocols
AccessionType
array scanning protocol
The scanning was performed according to the manufacturer’s protocol (Agilent technologies, A Method for Quantifying the performance of a DNA Microarray Scanner, Publication Number 5988-9498EN). (Parameters: Scanning hardware = DNA Microarray Scanner BA [Agilent Technologies], Scanning software = Feature Extraction Software [Agilent])
nucleic acid hybridization to array protocol
The hybridization experiment was performed according to the manufacturer's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Chamber type = OTHER: Robbins Scientific 1040-60-1AG, Quantity of label target used = 1, Mass unit = Micro gram, Tiny time unit = seconds, Volume unit = Nano litre, temperature = 65)
nucleic acid labeling protocol
The labeling experiment was performed according to the manufacturer's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Amplification = none, Mass unit = Micro gram)
nucleic acid labeling protocol
The labeling experiment was performed according to the manufacturer's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Amplification = none, Mass unit = Micro gram)
growth protocol
Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07–1.08) under a 16 h light/8 h dark regimen (40 ± 10 umol photons/m2/s) at 22C. (Parameters: start time = 3, time unit = weeks, min temperature = 22, temperature unit = C, media = soil)
nucleic acid extraction protocol
Total RNAs were isolated using the RNAiso Reagent (Takara, Japan). (Parameters: Extracted product = total_RNA, Amplification = none)
treatment protocol
The 3-week-old plants were grown for 6 days without watering. To obtain accurate results, we carefully raised single plants in Petri dishes, each containing an equal amount of soil. Soil moisture contents were calculated from soil dry weight. Under dehydration, on the sixth day, the soil moisture content was >10%.
treatment protocol
The plants were incubated at 22C under non-stress condition.