E-MEXP-3970 - Transcriptional response of Pseudomonas aeruginosa PAO1 to human host defense peptide LL-37
Released on 20 December 2013, last updated on 3 June 2014
In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa towards physiological concentrations of the major human host defense peptide LL-37 was investigated using microarrays. To this aim, three independent cultures of P. aeruginosa PAO1 were grown until mid-log phase in Mueller-Hinton broth and subsequently incubated with either sublethal LL-37 concencentrations (20 µg/ml) or without peptide for 2 h at 37 °C following RNA extraction and microarray analysis.
transcription profiling by array, co-expression, compound treatment design, in vitro
Human Host Defense Peptide LL-37 Stimulates Virulence Factor Production and Adaptive Resistance in Pseudomonas aeruginosa. Strempel N, Neidig A, Nusser M, Geffers R, Vieillard J, Lesouhaitier O, Brenner-Weiss G, Overhage J. PLoS One 8(12):e82240 (2013), Europe PMC 24349231