Title: Affymetrix CEL analysis. Description:
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
nucleic acid extraction protocol
Standard procedures were used to obtain total RNA (Qiagen) from the epididymal white adipose tissue of 15 day old wt and Ercc1-/- animals (n=4).
(Parameters: Extracted product = total_RNA, Amplification = none)
Epididymal white adipose tissue was dissected from 15 day old wt and Ercc1-/- animals (n=4).
(Parameters: time unit = seconds, temperature unit = C)