E-MEXP-3927 - Transcription profiling by array of yeast with homozygous CCC2 deletion grown with different concentrations of copper
Released on 2 September 2013, last updated on 3 June 2014
A 3 x 2 factorial design was used to elucidate the genome-wide transcriptional response to the deletion of yeast ortholog of Wilson and Menkes disease causing gene; CCC2, at changing copper levels. Homozygous deletion mutant of CCC2, which encodes Cu+2 transporting P-type ATPase required to export copper from the cytosol into the extracytosolic compartment, and the reference strain were cultivated in fully controlled fermenters in duplicates in glucose-rich defined medium containing three different levels of copper. The three different copper concentrations were selected such that; copper deficient condition, which was prepared by excluding the CuSO4.7H2O from the defined medium, low copper or adequate copper concentration, which is the standard amount of copper in defined medium (0.04 ?M) and high copper concentration (0.5 mM), which was able to restore respiration deficiency in ccc2?/ccc2? strain.
transcription profiling by array, co-expression, genetic modification design, growth condition design, replicate design
Transcriptional Remodelling in Response to Changing Copper Levels in Wilson and Menkes Disease Model of Saccharomyces cerevisiae. Ayca Cankorur-Cetinkaya, Serpil Eraslan and Betul Kirdar. Mol BioSyst (2013)