E-MEXP-3903 - microRNA profiling by array of human liver sinusoidal endothelial cells (LSECs) treated with IFN-a
Released on 17 May 2013, last updated on 3 June 2014
LSECs were grown in culture medium supplemented with 10% FBS (depleted of endogenous exosomes by overnight centrifugation at 100,000 g) and were treated with or without 1,000 U/ml IFN-a (PBL Interferon Source, Piscataway, NJ, USA) for 48 h. The exosomes from the cell culture supernatants were isolated by differential centrifugation as follow, at 300g for 10 min, 2,000g for 10 min, 10,000g for 30 min, and 100,000g for 70 min, washed once with PBS, and purified by centrifugation at 100,000g for 70 min. Total RNA of purified exosomes were extracted and Exiqon miRCURY LNA Expression Array were used for detection of mRNA and miRNA, respectively.
microRNA profiling by array, co-expression, compound treatment design, in vitro, stimulated type
Exosomes mediate the cell-to-cell transmission of interferon alpha-induced antiviral activity.