E-MEXP-3898 - Transcriptional profiling by array of Solenopsis invicta (fire ant) queens to identify genes responsible for modification of social rank during pleometrotic colony founding
Released on 21 August 2013, last updated on 3 June 2014
This experiment was performed to investigate the effect of the manipulation of social rank on gene expression. Fire ants newly mated queens were paired and placed in nesting chambers. After emergence of workers, queens’ behavior was monitored. Once the behavioral observation revealed the social rank of the two cofoundresses (winners and losers), queens were weighed again and re-paired with a different partner. We created the following three groups of queens: a) winner + winner (similar weight), b) loser + loser (similar weight), and c) winner + loser (different weights). Again, we monitored the behavior until the social rank of the newly coupled specimens was evident and we collected 4 new behavioral phenotypes in the same way as above: a) winners switched into losers (win/los), b) losers switched into winners (los/win), c) continuing winners (win/win) and d) continuing losers (los/los).
transcription profiling by array, co-expression, dye swap design, innate behavior design, loop design
Sociogenomics of Cooperation and Conflict during Colony Founding in the Fire Ant Solenopsis invicta. Manfredini F, Riba-Grognuz O, Wurm Y, Keller L, Shoemaker D, Grozinger CM. PLoS Genetics 9(8):e1003633 (2013), Europe PMC 23950725