4 protocols
hybridization protocol
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
array scanning protocol
Standard Affymetrix Scanning protocol. (Parameters: Scanning hardware = 418 [Affymetrix], Scanning software = MAS/GCOS/GREX [Affymetrix])
nucleic acid extraction protocol
Single cell suspensions were lysed in buffer RLT or Trizol and the RNA extracted following the RNeasy micro kit (Qiagen). The integrity of RNA was ensured by analysis of ribosomal 18S and 28S RNA intensity using an Agilent 2100 bioanalyser (Agilent Technologies). (Parameters: Extracted product = total_RNA, Amplification = none)