E-MEXP-3886 - Transcription profiling by array of Solenopsis invicta queens to identify genes associated with individual (haplometrotic) and group (pleometrotic) colony founding behavior
Released on 21 August 2013, last updated on 3 June 2014
Fire ant queens were collected immediately after a nuptial mating flight and split into two groups: paired queens (pleometrosis) and individual queens(haplometrosis). All the queens were provided with a nesting chamber consisting of a glass tube half-filled with water, which was covered by a cotton ball and a layer of dental plaster. Tubes were sealed with a loose cap to provide air flow. Specimens were reared in the dark at 28C, 70% relative humidity under claustral conditions (no food and no water) for 1 month. After the eclosion of the first batch of workers (minims), incipient colonies were provided with water, sugar water and frozen crickets. Glass tubes were set open in pencil boxes coated with Fluon to prevent escape. Queens were subsequently monitored daily until it was possible to identify the social rank of the two cofoundresses in pleometrotic couples.
transcription profiling by array, co-expression, dye swap design, innate behavior design, loop design
Sociogenomics of Cooperation and Conflict during Colony Founding in the Fire Ant Solenopsis invicta. Manfredini F, Riba-Grognuz O, Wurm Y, Keller L, Shoemaker D, Grozinger CM. PLoS Genetics 9(8):e1003633 (2013), Europe PMC 23950725
Sociogenomics of Cooperation and Conflict during Colony Founding in the Fire Ant Solenopsis invicta. Manfredini F, Riba-Grognuz O, Wurm Y, Keller L, Shoemaker D, Grozinger CM. PLoS Genetics 9(8):e1003633 (2013)